Defective Particles in BHK Cells Infected with Temperature-Sensitive Mutants of Vesicular Stomatitis Virus
نویسندگان
چکیده
منابع مشابه
Temperature-Sensitive Mutants of Vesicular Stomatitis Virus
Different temperature-sensitive mutants of vesicular stomatitis virus have been characterized in terms of their ability to induce synthesis of viral ribonucleic acid (RNA) in BHK-21 cells at 39 C (the restrictive temperature for these mutants). Mutants belonging to complementation groups I and IV (and probably II) did not induce actinomycin-resistant RNA synthesis in infected cells incubated at...
متن کاملMaturation of viral proteins in cells infected with temperature-sensitive mutants of vesicular stomatitis virus.
Maturation of viral proteins in cells infected with mutants of vesicular stomatitis virus was studied by surface iodination and cell fractionation. The movement of G, M, and N proteins to the virion bud appeared to be interdependent. Mutations thought to be in G protein prevented its migration to the cell surface, allowed neither M nor N protein to become membrane bound, and blocked formation o...
متن کاملTemperature-sensitive mutants of vesicular stomatitis virus; homology and nomenclature.
Reciprocal complementation experiments in mouse L cells and BHK-2I cells show that the complementing temperature-sensitive (ts) mutants of vesicular stomatitis virus (VSV), Indiana serotype, isolated from the HR wild-type strain, belong to groups I, III and IV (Flamand & Pringle, I97I). Since the homologies of ts mutants isolated from three different wild-type strains growing in different host ...
متن کاملDifferential inhibition of host protein synthesis in L cells infected with RNA - temperature-sensitive mutants of vesicular stomatitis virus.
The response of mouse L cells to infection with wild-type (wt) and temperature-sensitive (ts) mutants of vesicular stomatitis virus was monitored by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to delineate the synthesis of host cell and viral proteins. Experiments utilized transcriptase mutants of complementation group I (ts114 and ts13), a group IV mutant (ts44) that is restricte...
متن کاملPrimary in vivo transcription of vesicular stomatitis virus and temperature-sensitive mutants of five vesicular stomatitis virus complementation groups.
The process of adsorption and RNA transcription by vesicular stomatitis virus (VSV) in cells has been followed by using highly labeled preparations of VSV virions. The initial transcription process (primary parental transcription) is rapid and takes about 4 min to develop complete transcripts of the input genome. The optimal cell temperature for in vivo transcription is between 36 and 39.5 C, a...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1971
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.8.2.154-160.1971